Activities

We have perform immunohistological analysis of the new mutant mice model mrgprD-cre. The expression of ChR2 in mice under the control of the mrgprD promoter (green) is located below the immunolabelling for CGRP (red), a pattern diferent to other models. 

We have obtained data on the expression of EYFP in the spinal cord of mice expressing channelrhodopsin under the control of the TRPV1 promoter.

Today is the last day of Alejandro in the lab. We have made a lot of experiments and we are about to finish the analysis, hopefully we can publish these results soon.

Alejandro has found a new lab to continue with his whole cell recordings.

Today we have received the mrgprD-cre mice from MMRRC repository. These mice were cryopreserved as frozen embryos and we have received 2 males and 2 females to make a colony here.

We are starting a new year and new people is coming to the lab. Today Elsa will join to the group to make her intership for the studies of pathological anatomy and cytodiagnostics. She will make inmunohistological procedures to characterise the TRPV1-cre mice model by analyzing the expression of channelrhodopsin.

In February Araceli is going to start her degree Thesis and intership for her Sanitary Biology studies. Araceli is going to learn and work in electrophysiology with the animals models of optogenetics that we have.

Today we have received the new mutant line of TRPV1-cre mice. We have purchased 3 females and 2 males homozygous from the Jackson laboratories. We hope that we can start doing experiments on this new model in the new year.

Today Sara is going to start her master thesis in the lab. Sara is going to make behevioural studies in adult mice to test the antinociceptive effects of the new Kv7 channels opener compound 60. Her work is directly related with the project of Ingride.

Today we start our new grant to study the "Mechanisms of spinal plasticity mediated by nociceptive afferents". We are very excited to start this new project for the next 3 years. 

During the next 6 months we will have the visit of a PhD student from Italy. Ingride will stay in the lab working on pharmacological experiments with an Kv7 channel opener to test its effects on nocciceptive segmental transmission in the spinal cord in vitro.

During this semester we are going to incorporate David and Cristina to do their interships. David is in his last year of Sanitary biology. Cristina is enrolled in pathological anatomy and cytodiagnostics estudies. 

A new reseracher has been incorporated to the group. Alejandro is going to work in the lab for the next 2 years.

Welcome.

Today we received the male heterozygous Nav1.8-Cre mice kindly provided by Dr. Roza. We will do experiments with these mice in September, when we will have offspring after mating these males with females homozygous for rhodopsin-2.

Finally, the homozygous Gad2-Cre mice needed for the experiments arrived today. In two weeks we will make the mating to obtain mice expressing rhodopsin-2 in inhibitory spinal neurons characterized by the expression of the gad2 enzyme. Before summer we will make a first series of experiments with these mice.

We had finally incorpored the new parts of the equipment, we have a bath chamber adjusted to our needs and everything is fine now. LETS GO.

We have mounted the new equipment. We are trying to arrange all the parts. Soon we are going to start the experiments to test if everything can work fine. 

Today, Marta Jaén and Juan Pedro García arrive to the laboratory to join us in this project. WELCOME.

We have started to configure the new electrophysiological setup necessary to record from specific populations of neurons labelled by the genetic expression of fluorescent proteins. Then we will start the administrative procedure to order the different components and finally mount the equipment.

We are currently working in finding the best way to obtain the mutant mice necessary for the proposed studies. The breeder of these mice maintain the animals with one oportunistic microorganism that is not accepted in our animal facility, so we are considering several options to receive the mice in adequate conditions.